Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221332_ejno CTTTACCTTGACTTCTCCCAGCCATGGCCCTG(+ATATAT)GATCCCCCAGACAATCGTCTTCGCCCACCGC NGG 10 8 17 8 24 0 100 5 10
    240606221330_vi9b GGGGAGTCAGAATGGGAAAGATGCATACGCTG(+ATATAT)GATCCTTCAGAAATTTACAGGGCAGGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606221329_m57t AACACTGAGCCCTGCCCTGTAAATTTCTGAAG(+ATATAT)GATCCAGCGTATGCATCTTTCCCATTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240606221327_j434 ATGTGATTCACGGCCTGATGCTGTTCGCTAGG(+ATATAT)GTACCTCAAACACGTGACATAGGCGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606221326_q4x2 TCGAGGCTGGGCGCCTATGTCACGTGTTTGAG(+ATATAT)GTACCCTAGCGAACAGCATCAGGCCGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606221324_uktx AGCAAGTTTTGCTGCTAGGACTAAGGATGGTG(+ATATAT)GTACCCAAAGTACGAACGTACAAAGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606221322_n92o CTTGCTGGAGTCTTTGTACGTTCGTACTTTGG(+ATATAT)GTACCACCATCCTTAGTCCTAGCAGCAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606221320_yl6c ACTGGAACCTTGCTCTTGACGGCCACTCCCAG(+ATATAT)GATCCTGTCTCCATGCCAAGCATCTTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606221318_a8l7 TTAGCCCTGGAAGATGCTTGGCATGGAGACAG(+ATATAT)GATCCTGGGAGTGGCCGTCAAGAGCAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606221317_vqls GAGTCAATGTCATGGTAGTCTGCAGGGGGAAG(+ATATAT)GATCCCTTGGTACAAACTTCATGTCTTTAAA NGG 10 8 17 8 24 0 100 5 10