Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221219_oefl GGCTTCTTTGACTGTCAAGTGATTAACATATG(+ATATAT)GTACCCGGTGTCAGAACATTTAAGCGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606221217_bz0e TCTTAAAATACAAGCAGGTATCTCATCTGAAG(+ATATAT)GTACCCTCACAGCTTCCTCGCAACAGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606221215_wg8v CCTGTCCCTGCTGTTGCGAGGAAGCTGTGAGG(+ATATAT)GTACCTTCAGATGAGATACCTGCTTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606221213_9xpt CCTGATGCTACAGAAGCCAGATGAGGGGACTG(+ATATAT)GATCCCCTGGAACTGGAGGTACAGATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606221211_ozpb GCTCACAGCCATCTGTACCTCCAGTTCCAGGG(+ATATAT)GATCCAGTCCCCTCATCTGGCTTCTGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606221208_x3ae GCCACGCCTCGCTCTCTGTGTACAGCAATGGG(+ATATAT)GATCCAGGAAGCAGGATGGGCCAGGAACCCG NGG 10 8 17 8 24 0 100 5 10
    240606221206_r62k GCCTTCGGGTTCCTGGCCCATCCTGCTTCCTG(+ATATAT)GATCCCCATTGCTGTACACAGAGAGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606221205_q8kr AGACTATGTGCTACCTTCACTTGCTAATGCAG(+ATATAT)GATCCAAAGAATGCAGGCAAACCCTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606221203_bbat GCTACATTTGTAGGGTTTGCCTGCATTCTTTG(+ATATAT)GATCCTGCATTAGCAAGTGAAGGTAGCACAT NGG 10 8 17 8 24 0 100 5 10
    240606221202_8ny5 TAAATGGGACAAAGACATAGGCAAAAAACATG(+ATATAT)GATCCTAAGTAGAATCAGATATTTATCTCTT NGG 10 8 17 8 24 0 100 5 10