Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221143_2jc5 ATTGCTTTTAAGTAACCTCTGGTCTTTACTAG(+ATATAT)GATCCCCTCAGTTTGTCTTGTCATGTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606221142_y27t AAAAGGAAAAAGAATTAGACTGGATAGAAGTG(+ATATAT)GTACCCAACACTGGGACTCAATTTTTATTTT NGG 10 8 17 8 24 0 100 5 10
    240606221140_sb3a AATTTAAAATAAAAATTGAGTCCCAGTGTTGG(+ATATAT)GTACCACTTCTATCCAGTCTAATTCTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606221139_1q5g AGATTTCAAGTAAAACTGAGCTGAGATACAAG(+ATATAT)GATCCCCAAAAAAGTTCTTAGCTTATAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606221137_p1e4 ACTTAGTTTTATAAGCTAAGAACTTTTTTGGG(+ATATAT)GATCCTTGTATCTCAGCTCAGTTTTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240606221135_si77 AGTAGCCATTCTCTAAATGAACTGTAGGAGTG(+ATATAT)GATCCTTAGGGAAGGGAGCGCAGGTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240606221133_wkk5 AGTTCTGAATGACCTGCGCTCCCTTCCCTAAG(+ATATAT)GATCCACTCCTACAGTTCATTTAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240606221131_uxoz ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+ATATAT)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606221130_34pc TGGCATTGCAGTTCACTTCCAAAGCCGTCTCG(+ATATAT)GATCCTGAGGCCCTGCGTTAGACAGGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606221127_6tlb ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+ATATAT)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10