Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221200_zhuc AGAAAAAGAGATAAATATCTGATTCTACTTAG(+ATATAT)GATCCATGTTTTTTGCCTATGTCTTTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606221158_g5cm ATACTTTCCACATCTGGGGAATGTAAGCTAGG(+ATATAT)GTACCTCATCTGGGATTTATCTTATTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606221157_2wxl GATCTGGAAGAATAAGATAAATCCCAGATGAG(+ATATAT)GTACCCTAGCTTACATTCCCCAGATGTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606221155_srn0 AGTCCACAGGATAAAAGAATGTGACCCCAAAG(+ATATAT)GATCCTTCTGTAGCTGATCAAGCCACCCTAA NGG 10 8 17 8 24 0 100 5 10
    240606221154_s41h ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+ATATAT)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606221152_gb3y TGTGCCACAGGATTATGCATAGCCACCGTTAG(+ATATAT)GTACCACAGCTGTTACACAGAGCTTTAAACT NGG 10 8 17 8 24 0 100 5 10
    240606221151_ib97 CAATCAGTTTAAAGCTCTGTGTAACAGCTGTG(+ATATAT)GTACCTAACGGTGGCTATGCATAATCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606221150_y9i6 ATTGAAGTTTTTTTCCATATAAAGATAGATAG(+ATATAT)GTACCATAAAATCAAGTGACTGCTTTTATAA NGG 10 8 17 8 24 0 100 5 10
    240606221148_w23u CTCTGTTATAAAAGCAGTCACTTGATTTTATG(+ATATAT)GTACCTATCTATCTTTATATGGAAAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240606221146_xldr AACATATACCACATGACAAGACAAACTGAGGG(+ATATAT)GATCCTAGTAAAGACCAGAGGTTACTTAAAA NGG 10 8 17 8 24 0 100 5 10