Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221105_5s6b CCACACACCCTGCACAGTGCTGCCCCGGGGAG(+ATATAT)GTACCAAAGCCTACACCTACAGAGACTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606221101_x198 CTGTTTCCCTAAACTCCATCCTTTCTTCCCAG(+ATATAT)GATCCTGCCCTTCATAGAGGGAACAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606221100_093h TGGAGCTACCCTGTTCCCTCTATGAAGGGCAG(+ATATAT)GATCCTGGGAAGAAAGGATGGAGTTTAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606221058_1cg5 CTGGGGCTTCTCTCCACTTTCTGGCAAGGAAG(+ATATAT)GTACCAGGGTCACAGACTCCCACACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606221056_mc41 TACTTGAGACTGTGTGGGAGTCTGTGACCCTG(+ATATAT)GTACCTTCCTTGCCAGAAAGTGGAGAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606221053_cd1c CCTGTCAGTGAGGGGGGTTGACTCCTGTGTGG(+ATATAT)GTACCATTGGGGAAGGATGGATTTATCACAC NGG 10 8 17 8 24 0 100 5 10
    240606221051_w35u GGAGAGTGTGATAAATCCATCCTTCCCCAATG(+ATATAT)GTACCCACACAGGAGTCAACCCCCCTCACTG NGG 10 8 17 8 24 0 100 5 10
    240606221050_upap AGACCAAGAACAGCAGAAGCTCACAGAGTGAG(+ATATAT)GATCCAGGGAAGCCTTGGCAGGCTGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606221047_1kww CCGCTTGCCCACAGCCTGCCAAGGCTTCCCTG(+ATATAT)GATCCTCACTCTGTGAGCTTCTGCTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606221044_s5u7 CACCTGGGAACCACTCGACTACTGACCCAAAG(+ATATAT)GTACCCTCCCCAGGGCTGCTCAGGACCAGTG NGG 10 8 17 8 24 0 100 5 10