Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221003_fxrn GGAATGCTGGTGCTCAGGTTGGAGAAGCTAGG(+ATATAT)GATCCCAGGGGCAGCTGCCTGGTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606221002_4d5i GAGCCCTTTATTGGCTTGGATTAGCTGTGTAG(+ATATAT)GATCCAGGGACTGTCCTCGTCCTCGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606220959_blhm GCAAGCCAACCCGAGGACGAGGACAGTCCCTG(+ATATAT)GATCCTACACAGCTAATCCAAGCCAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606220958_daxa AATGTCTCCTCTCCAGTAGCTCCACAGAGGTG(+ATATAT)GTACCTCAGCCAAAGCCATTCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240606220957_6qbz AGGTTCATGGGTTAGGAATGGCTTTGGCTGAG(+ATATAT)GTACCACCTCTGTGGAGCTACTGGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606220956_vzej AAAAAGCTTTATTAAAAGTTAGCCCTTACAAG(+ATATAT)GATCCTATTAAAATATACACATTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240606220954_fo81 TGCATTTATTTAAAATGTGTATATTTTAATAG(+ATATAT)GATCCTTGTAAGGGCTAACTTTTAATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606220952_x11z TCTAATCTCTGTCAAAAGGGAGGTTAGGAAAG(+ATATAT)GTACCCCCTCCCTTGTAAACACTGAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606220950_9en6 AGATCTGTATTTCAGTGTTTACAAGGGAGGGG(+ATATAT)GTACCTTTCCTAACCTCCCTTTTGACAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606220948_bzg4 CTGCCAGAGGCTTGCCCAAGAAAAGGAGTCGG(+ATATAT)GTACCTGTCAGATGCTACCTGGAGGGTCAGG NGG 10 8 17 8 24 0 100 5 10