Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220916_cz0y TCAAACTCTGGGCTCAGTTCTCAGGCCACAGG(+ATATAT)GTACCCAGCCTGCACCTAGCAAGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606220913_ei6e TCCCGCTCTGGGACTTACTGGGGAGGGGGCGG(+ATATAT)GTACCAGTGTATGGAGTAACAAGACATCAGC NGG 10 8 17 8 24 0 100 5 10
    240606220912_u46s CGAAGGCTGATGTCTTGTTACTCCATACACTG(+ATATAT)GTACCCGCCCCCTCCCCAGTAAGTCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606220910_gvph GGCTTGCCTGTCGGTCAAGAGACCCTCGGATG(+ATATAT)GATCCCAGGACTGTAAAATATAAATAAATAA NGG 10 8 17 8 24 0 100 5 10
    240606220907_q0s1 TATTTTTATTTATTTATATTTTACAGTCCTGG(+ATATAT)GATCCATCCGAGGGTCTCTTGACCGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606220906_vgx1 CAATGCTGTTCGCTAGATTTTGCAACCACCTG(+ATATAT)GATCCTGTTTGCCGATACCCCTCATCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240606220904_e6sx CAGAAATCCGGATGAGGGGTATCGGCAAACAG(+ATATAT)GATCCAGGTGGTTGCAAAATCTAGCGAACAG NGG 10 8 17 8 24 0 100 5 10
    240606220903_nit7 CTGTGTCTCATATTTATATCATAAAAACATTG(+ATATAT)GATCCCTATAACTTCAAAGTCGTGTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606220900_y72z AGTGAAGAAGCACACGACTTTGAAGTTATAGG(+ATATAT)GATCCAATGTTTTTATGATATAAATATGAGA NGG 10 8 17 8 24 0 100 5 10
    240606220859_zyhs AGGTTTCTAATTGCCCAGCTGCTAGGTAACTG(+ATATAT)GTACCTTTTAAAGCCCCCTGTAATTTTCCTT NGG 10 8 17 8 24 0 100 5 10