Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220805_fpg1 TAGAGGCTCGTCTGCACACCCAGGCTTCAGGG(+ATATAT)GTACCAGCTCATGGACTTTCTGGTTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606220803_ecib TGGCCCTTGCAGTACTGTAAGATTGTGATGGG(+ATATAT)GATCCTTTTGTTTGTTTGACTAATGTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606220801_58vu ATTCTGAGAGACATTAGTCAAACAAACAAAAG(+ATATAT)GATCCCCATCACAATCTTACAGTACTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606220800_ca2d GCATTAATATCATTATTGCTGTGCCATATTTG(+ATATAT)GATCCAAAGTTGCTCCAGGTTAATCCAACTT NGG 10 8 17 8 24 0 100 5 10
    240606220755_h83r GTATAAAGTTGGATTAACCTGGAGCAACTTTG(+ATATAT)GATCCAAATATGGCACAGCAATAATGATATT NGG 10 8 17 8 24 0 100 5 10
    240606220753_6ml6 GGGCCATTCAGTCCTATTTCAATGGCAGTGTG(+ATATAT)GATCCCTGCTCTTATCTAAGTATCTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606220750_pkyi TGTATACACCCAGATACTTAGATAAGAGCAGG(+ATATAT)GATCCACACTGCCATTGAAATAGGACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606220747_or4m GGGATTGTTCCAAGATACTGAAGATTTGGTGG(+ATATAT)GTACCATAGCATCTTTTGACTACTGATCTGT NGG 10 8 17 8 24 0 100 5 10
    240606220744_8qv6 GGTAGACAGATCAGTAGTCAAAAGATGCTATG(+ATATAT)GTACCCACCAAATCTTCAGTATCTTGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606220742_tkpt TAAAGTCACTTATAGATACTCAGAAGGAAAAG(+ATATAT)GTACCCCAATATTCCAATAGTATCGGTACAT NGG 10 8 17 8 24 0 100 5 10