Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220621_58t1 CGCCATATTCAGGTCTAAGTGCCTGCTCTCGG(+ATATAT)GTACCGTGAGCAAACTTGCCCTTTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606220618_pcs9 CCAGCTACTGCATCCAGTGGCCTGGGGCTCAG(+ATATAT)GATCCATTTTCTACATTCATAAGAATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606220616_7zzf GGGCATGGGCATTCTTATGAATGTAGAAAATG(+ATATAT)GATCCTGAGCCCCAGGCCACTGGATGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606220613_2oui TATAGGACTTGATGAAATTAAGCAAGGTCCTG(+ATATAT)GATCCCAGAAACAGGTCCACAGGTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240606220611_f13s GCATACCGAAGAACCTGTGGACCTGTTTCTGG(+ATATAT)GATCCAGGACCTTGCTTAATTTCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606220609_7dld CGATGGCTCTATTAATACACACACGGTGACAG(+ATATAT)GTACCCTATTTAGACTAAGGCTACTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606220606_biz2 GAGTGTGTTCTAGTAGCCTTAGTCTAAATAGG(+ATATAT)GTACCTGTCACCGTGTGTGTATTAATAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606220603_rv9c GATACATCTACTCAGCATAGGAGAGCCCTGGG(+ATATAT)GTACCACTTACAGGCTATCTAGAATAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606220600_ntf1 GCTGTGGAACTATTCTAGATAGCCTGTAAGTG(+ATATAT)GTACCCCAGGGCTCTCCTATGCTGAGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606220557_z69y CATGTGTGCTCGTTGTCTTAAGTCCCTGGAGG(+ATATAT)GTACCACACTGCTTTCATCCTGTCCCCTTGG NGG 10 8 17 8 24 0 100 5 10