Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220453_is45 AATGCTGTGTGTCCACACAGGCTGCTTTCCTG(+ATATAT)GATCCTGCGGTACCACAGGATGTTGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606220451_iva6 TTGACTGAGTAAAGGAGAGGTGGTTTCTATGG(+ATATAT)GATCCTTTCTGTCTGCTGGGGGACATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606220450_5ka2 CCAATGCCCCATGTCCCCCAGCAGACAGAAAG(+ATATAT)GATCCCATAGAAACCACCTCTCCTTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240606220448_r5ni AGACTGAGGCTTGTGGCCATTGTGCCAAATGG(+ATATAT)GTACCTTGCTGAAACACAGGGTTACAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606220446_8wu4 GGGGGCACACTGTAACCCTGTGTTTCAGCAAG(+ATATAT)GTACCCATTTGGCACAATGGCCACAAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606220444_u3w8 AGCTCAATGTGAGCAGGGGCTGCCGACTGAGG(+ATATAT)GTACCTGGCACATATCTTTTTCCATAACACA NGG 10 8 17 8 24 0 100 5 10
    240606220442_1n2m TCATCTGTGTTATGGAAAAAGATATGTGCCAG(+ATATAT)GTACCCTCAGTCGGCAGCCCCTGCTCACATT NGG 10 8 17 8 24 0 100 5 10
    240606220440_v669 AGTGACATATAACGCATGCCACATTAATGTGG(+ATATAT)GATCCATCCACAGTGACTTAGGAAAGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606220437_vwiw GTTAGTCTTCCTTTCCTAAGTCACTGTGGATG(+ATATAT)GATCCCACATTAATGTGGCATGCGTTATATG NGG 10 8 17 8 24 0 100 5 10
    240606220435_8flh GGTTCCTGGTTCCTTCTGCTGATCCACACTGG(+ATATAT)GATCCAGCCCTCCTTTGCCTCAGCTGGTTTG NGG 10 8 17 8 24 0 100 5 10