Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220433_rk13 AGCCCCAAACCAGCTGAGGCAAAGGAGGGCTG(+ATATAT)GATCCCAGTGTGGATCAGCAGAAGGAACCAG NGG 10 8 17 8 24 0 100 5 10
    240606220432_r1vm AAGCACCTCTTGTCTGCTGTAGTTTCAGTCAG(+ATATAT)GATCCAGCTCATTTTCCATGGACCTAACTGG NGG 10 8 17 8 24 0 100 5 10
    240606220430_dhzv CTTGCCCAGTTAGGTCCATGGAAAATGAGCTG(+ATATAT)GATCCTGACTGAAACTACAGCAGACAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606220428_4apl CCTGATCCTTTGGGCTCTCTGGAGGGTTCTGG(+ATATAT)GTACCTAACCCTCCTCTCTGATCCACCCACC NGG 10 8 17 8 24 0 100 5 10
    240606220427_m9kb CCCAGGGTGGGTGGATCAGAGAGGAGGGTTAG(+ATATAT)GTACCCAGAACCCTCCAGAGAGCCCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606220425_2o2h GGCTACTGCAAGTCTGGAATTTTCTCATTGGG(+ATATAT)GATCCATGAGGGTGCTTGCTTGTGTCATTCG NGG 10 8 17 8 24 0 100 5 10
    240606220423_9778 AGTGGCGAATGACACAAGCAAGCACCCTCATG(+ATATAT)GATCCCCAATGAGAAAATTCCAGACTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606220422_8rla AGGTCCTGCTTCTGCTCAGCAGCTTGAGGGAG(+ATATAT)GTACCAGTGCCTCATTTTGTCTCTATCGGTT NGG 10 8 17 8 24 0 100 5 10
    240606220420_94wl CACAGAACCGATAGAGACAAAATGAGGCACTG(+ATATAT)GTACCTCCCTCAAGCTGCTGAGCAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606220417_1hq6 CTCTGCCAGCCTGCTCTGTTCCTCTGTCCCTG(+ATATAT)GATCCTCACTGGCAGCAGCTGTTACGCATTG NGG 10 8 17 8 24 0 100 5 10