Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220327_itr2 ATGCAGGCTTCCAGGACATTCTCTTCCATCAG(+ATATAT)GTACCCGAGGAAGCACTGGAAAGAGAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606220325_vpcl CCCAAACTTACTCCCCCCTAAAGGAGAAAGAG(+ATATAT)GATCCAGGAGGCTCAGTCCTGATGGAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606220323_nx5j TGTGATCCTCTCCATCAGGACTGAGCCTCCTG(+ATATAT)GATCCTCTTTCTCCTTTAGGGGGGAGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606220321_1xnd GCCGTCGGAGTTCCCCAAATCCAGTGGGGGTG(+ATATAT)GATCCACAATGGATCTATTCTACCTCCATAG NGG 10 8 17 8 24 0 100 5 10
    240606220320_b0hm TGGACCTATGGAGGTAGAATAGATCCATTGTG(+ATATAT)GATCCACCCCCACTGGATTTGGGGAACTCCG NGG 10 8 17 8 24 0 100 5 10
    240606220318_vgni TTTGGCCAGGTCAGCCAGGCCTGTTTTCACAG(+ATATAT)GATCCTAACAGGTGATCAGTGAGAAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240606220317_kxw7 GCTTCTTTAGCTTCTCACTGATCACCTGTTAG(+ATATAT)GATCCTGTGAAAACAGGCCTGGCTGACCTGG NGG 10 8 17 8 24 0 100 5 10
    240606220315_8n84 TAGAAGAAGATGGAGACACTGTGTCCTAAGAG(+ATATAT)GATCCTGTACCCCAGGCTAGGGTAATTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606220313_yaq8 CTGGGGGAGAATTACCCTAGCCTGGGGTACAG(+ATATAT)GATCCTCTTAGGACACAGTGTCTCCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240606220312_pktl GTCCAAAATCTTTCTGGCTAGCCAGTCAGTTG(+ATATAT)GATCCCAAGTCCTTTGGGGTAGCATGACTCT NGG 10 8 17 8 24 0 100 5 10