Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220416_c8mb TGCTCCAATGCGTAACAGCTGCTGCCAGTGAG(+ATATAT)GATCCAGGGACAGAGGAACAGAGCAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606220414_a1jj CTAGCCACACCTGTAGGGCAAGGTCACTGCAG(+ATATAT)GTACCTCCCTGTCATTAGGCAGTGGACTTCG NGG 10 8 17 8 24 0 100 5 10
    240606220413_vaz3 CTTCCCGAAGTCCACTGCCTAATGACAGGGAG(+ATATAT)GTACCTGCAGTGACCTTGCCCTACAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606220411_3bhe ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCAATATCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606220410_tmgg GCTGTCTTCAGACACACCAAAAGAGGATATTG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606220408_c3xc CGCCTCCCTGGTGATGCCACCTAGGAGGGTTG(+ATATAT)GATCCTGGACTCCAGCCCACCTTCTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606220406_xxeo CTAGGCCAGAGAGAAGGTGGGCTGGAGTCCAG(+ATATAT)GATCCAACCCTCCTAGGTGGCATCACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606220405_eacp CCCGAGAGCCACAGGCTGTGTCCATGAACTTG(+ATATAT)GATCCCTGAGCACTTGAGAATGAACTCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606220403_63gs GAACCTCTTGAGTTCATTCTCAAGTGCTCAGG(+ATATAT)GATCCAAGTTCATGGACACAGCCTGTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606220401_8n11 GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+ATATAT)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10