Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220533_b4t6 GTCCAATAGGATGGGGAAGGCCCAAACATAAG(+ATATAT)GATCCCGGTGAGTCAGGCAAGGCTGGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606220531_ppbb CTGAGATTACAGAAGTACCCAGAAGTGTCTGG(+ATATAT)GATCCCTGTCACATCTGTGTCATGTTGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606220529_7sol TTCAAATAACAACATGACACAGATGTGACAGG(+ATATAT)GATCCCAGACACTTCTGGGTACTTCTGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606220528_mcf4 TGATAGCCAACCTTGGTTTAGGCATCTCTCAG(+ATATAT)GTACCTACAATTTCCCAAGTTCCAATAAATT NGG 10 8 17 8 24 0 100 5 10
    240606220525_64u1 AACAGAATTTATTGGAACTTGGGAAATTGTAG(+ATATAT)GTACCTGAGAGATGCCTAAACCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606220523_vwnj GACAAAATAGCCCATGGAAGCACAGTGAAGGG(+ATATAT)GTACCCTGACAGACAGATGAGCCACAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606220522_nndo TTGAAGGGCCTGTGGCTCATCTGTCTGTCAGG(+ATATAT)GTACCCCTTCACTGTGCTTCCATGGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240606220520_2d2k CAGATGCCCTGGAACTGGAGTTACAGACTGGG(+ATATAT)GATCCTCTGGAAGAGCAGCCAAGGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606220518_oki2 AGTGGTTAAGAGCCTTGGCTGCTCTTCCAGAG(+ATATAT)GATCCCCAGTCTGTAACTCCAGTTCCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606220515_c6ub TTCACACTAAGTAAGGGTTAAACCAGTCCCTG(+ATATAT)GATCCTTAGTGAACTATAGAGGAGGGAGTAC NGG 10 8 17 8 24 0 100 5 10