Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220646_twsx GTGGGGCCGCCCGCACCTGCTGCATCGGGAAG(+ATATAT)GATCCTTGGAGGGGAGTATAAACAGGACGAA NGG 10 8 17 8 24 0 100 5 10
    240606220643_d2fo CCTGGGTGGAAACACTGCCTTGTAGCTCTGGG(+ATATAT)GTACCAGCCACAGATGATCCCTCTTGTACAA NGG 10 8 17 8 24 0 100 5 10
    240606220641_arkm TTTATTTGTACAAGAGGGATCATCTGTGGCTG(+ATATAT)GTACCCCAGAGCTACAAGGCAGTGTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606220638_fjxd GGTGGGGACATAAAGGCCTCCTGTGTGGCCTG(+ATATAT)GTACCTGAGATCTGAGGTTGTGCAGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240606220637_1sss CCGTCCTGATTCTGCACAACCTCAGATCTCAG(+ATATAT)GTACCAGGCCACACAGGAGGCCTTTATGTCC NGG 10 8 17 8 24 0 100 5 10
    240606220633_1shi ACCCGTTTGGCAGGGGCTCTAGTTGAGGGTTG(+ATATAT)GTACCCCAAGGAAGGATCATGATCGGCCGGA NGG 10 8 17 8 24 0 100 5 10
    240606220631_1kp3 AGGAGTCCGGCCGATCATGATCCTTCCTTGGG(+ATATAT)GTACCAACCCTCAACTAGAGCCCCTGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606220629_3jim CTATGAAAACCTTCCCTTTGACAGTTCAGTTG(+ATATAT)GATCCAGCTCCTGCTAGCCACTGAATTAATT NGG 10 8 17 8 24 0 100 5 10
    240606220626_km9a ACACAAATTAATTCAGTGGCTAGCAGGAGCTG(+ATATAT)GATCCAACTGAACTGTCAAAGGGAAGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606220624_makp TCTGTCTTCATAAAAGGGCAAGTTTGCTCACG(+ATATAT)GTACCCGAGAGCAGGCACTTAGACCTGAATA NGG 10 8 17 8 24 0 100 5 10