Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220713_29cw AGGAAGTCAGGACAAGATCTCAAACACAGCAG(+ATATAT)GATCCTAGATGCAGGGGCTGAAGCAGAAACC NGG 10 8 17 8 24 0 100 5 10
    240606220708_i5jf TCCTCCAACCACCTACAACTCCATCTCCAGGG(+ATATAT)GATCCAATGCCTTCTTCTGGTCTTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606220705_27ay CAGGTGCCTGCAAAGACCAGAAGAAGGCATTG(+ATATAT)GATCCCCTGGAGATGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606220702_gkej CTTAGCATTTTTACCTGCTGTTTGGCTCTGTG(+ATATAT)GTACCAAGTCATGGGAATTTCTCTCGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606220700_px9r CATTTGACCCCGAGAGAAATTCCCATGACTTG(+ATATAT)GTACCACAGAGCCAAACAGCAGGTAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606220658_huv7 AAGCATTCCCAGAGAGCAGTTGACTAGCGGGG(+ATATAT)GATCCTTTAAAGGGCCAGGCACACAAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606220656_gjj1 ATGATTTCTTTTGTGTGCCTGGCCCTTTAAAG(+ATATAT)GATCCCCCGCTAGTCAACTGCTCTCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606220653_ov9z GTATTTTCAGAGTCAGTTGACTAGCCATGGGG(+ATATAT)GATCCTTTAAGGGGCCAGGCACGCACATCAT NGG 10 8 17 8 24 0 100 5 10
    240606220651_wp03 GTAGAATGATGTGCGTGCCTGGCCCCTTAAAG(+ATATAT)GATCCCCCATGGCTAGTCAACTGACTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606220648_6p8e CCTGATTCGTCCTGTTTATACTCCCCTCCAAG(+ATATAT)GATCCTTCCCGATGCAGCAGGTGCGGGCGGC NGG 10 8 17 8 24 0 100 5 10