Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220825_fine TGTTAATTTTCAGTGTTGTTGGATTCTTGGTG(+ATATAT)GTACCTTGTGTTTTCATAATTATGTCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606220823_aze0 AAATTACATAAACTACTGGAAAACCAGTGAAG(+ATATAT)GTACCAGGTCAATAGATTAAAAGACTATACA NGG 10 8 17 8 24 0 100 5 10
    240606220820_fs12 CGTATTGTATAGTCTTTTAATCTATTGACCTG(+ATATAT)GTACCTTCACTGGTTTTCCAGTAGTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240606220819_lor1 CCTTAGGCAGTGCCTGCTACTCCAAAGGGGAG(+ATATAT)GTACCGTTAGTGTGCAGGAATGAGACACTTG NGG 10 8 17 8 24 0 100 5 10
    240606220817_tupl CCTTCCAAGTGTCTCATTCCTGCACACTAACG(+ATATAT)GTACCTCCCCTTTGGAGTAGCAGGCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606220815_fp29 GTGCAGGAATGAGACACTTGGAAGGCAGATGG(+ATATAT)GATCCAAGAGAAAGGCATACTTGGTAAACTA NGG 10 8 17 8 24 0 100 5 10
    240606220813_lu16 CAATATAGTTTACCAAGTATGCCTTTCTCTTG(+ATATAT)GATCCCATCTGCCTTCCAAGTGTCTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606220811_pyrv CATCTACTTTGCCTTTTTGTGCAGGTTATATG(+ATATAT)GATCCTGCACCGCTCATTACAAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606220809_hai0 AAACGTGCAGACCTTTGTAATGAGCGGTGCAG(+ATATAT)GATCCATATAACCTGCACAAAAAGGCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606220808_cmba GAGTCCAGCATAACCAGAAAGTCCATGAGCTG(+ATATAT)GTACCCCTGAAGCCTGGGTGTGCAGACGAGC NGG 10 8 17 8 24 0 100 5 10