Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220931_cjzz CCAGGTGTTCTCGCCCTGCCAGCTCCTCCTGG(+ATATAT)GTACCCTCCTTGCTTGGCTCCTTCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606220930_yvsj CTCTACTCTTTCTTGCCATCGCCCCTGGCATG(+ATATAT)GATCCAGAGACTCAGGCTACAACAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606220929_sxvs AAAGTGGCTTCCTGTTGTAGCCTGAGTCTCTG(+ATATAT)GATCCATGCCAGGGGCGATGGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606220927_eebd AGCCAAGCTGCGTGAGTGGAGTGTGGGGAATG(+ATATAT)GATCCCTTGGGGGCTACAGAAAGAGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606220925_1viv AAGAGCCTTCACTCTTTCTGTAGCCCCCAAGG(+ATATAT)GATCCATTCCCCACACTCCACTCACGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606220923_mnq9 ACACACACAGAGACCTGCCCCTGCTGTGGTTG(+ATATAT)GATCCAATAAACTTTCTGGAGGCATAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606220922_51pl GCCCACAAGCTATGCCTCCAGAAAGTTTATTG(+ATATAT)GATCCAACCACAGCAGGGGCAGGTCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606220921_9xwf CCCCAGCCTTGAGGTTCAACTGCCTAGGCTCG(+ATATAT)GTACCCGCAGCATCCCTTGCCTATTTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606220919_v831 GAAAAGTTAAAAATAGGCAAGGGATGCTGCGG(+ATATAT)GTACCGAGCCTAGGCAGTTGAACCTCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606220917_8gd6 CAGCTTCAGCAACCTTGCTAGGTGCAGGCTGG(+ATATAT)GTACCCTGTGGCCTGAGAACTGAGCCCAGAG NGG 10 8 17 8 24 0 100 5 10