Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221042_ly0u CTGCACACTGGTCCTGAGCAGCCCTGGGGAGG(+ATATAT)GTACCTTTGGGTCAGTAGTCGAGTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606221039_1bp9 CACCTGGGAACCACTCGACTACTGACCCAAAG(+ATATAT)GTACCCTCCCCAGGGCTGCTCAGGACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606221037_znkh CTGCACACTGGTCCTGAGCAGCCCTGGGGAGG(+ATATAT)GTACCTTTGGGTCAGTAGTCGAGTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606221035_fn2c TGTTTGCTGTGATTGCTTGACAATGTGAGTTG(+ATATAT)GATCCCTGGTAGGAGAGAAGTAGAAGTAACT NGG 10 8 17 8 24 0 100 5 10
    240606221033_0nq6 TGTGGAGTTACTTCTACTTCTCTCCTACCAGG(+ATATAT)GATCCAACTCACATTGTCAAGCAATCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606221031_p8dp GCTACCCTCCCTTACTGCCATCCACACCCATG(+ATATAT)GTACCCATGGAAACATAGCCACCCTCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606221028_0eu0 GGCAGGAGGGGAGGGTGGCTATGTTTCCATGG(+ATATAT)GTACCATGGGTGTGGATGGCAGTAAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606221026_srzs GAATATTAGATATTTTTTCCATGGGGTCAAAG(+ATATAT)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606221025_7x90 CCCTATGTTTCCACTCGCAATCATATACTTAG(+ATATAT)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606221023_csis GTGGGTGGATACTGAACAGAAGCAGACAGAGG(+ATATAT)GTACCATTCTGTAACGCCCTGTTTATACACC NGG 10 8 17 8 24 0 100 5 10