Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221021_rgts CCTTTGGTGTATAAACAGGGCGTTACAGAATG(+ATATAT)GTACCCTCTGTCTGCTTCTGTTCAGTATCCA NGG 10 8 17 8 24 0 100 5 10
    240606221020_s4xt GGCCCAGTCCCAGGAACTGTGTCTGTGCATAG(+ATATAT)GATCCGGCTCTGCCCCGGGCCCGTCAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606221018_d8db CCCTCTTCATTGACGGGCCCGGGGCAGAGCCG(+ATATAT)GATCCTATGCACAGACACAGTTCCTGGGACT NGG 10 8 17 8 24 0 100 5 10
    240606221016_rqm7 GGCCTGGCTTTCGCAGGAGGGTCTGGCAGCTG(+ATATAT)GATCCATGTCTGTCCACCCACGCGGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606221015_4fie AGGAAGTTCCCCCGCGTGGGTGGACAGACATG(+ATATAT)GATCCAGCTGCCAGACCCTCCTGCGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606221013_13n6 GGGACCATCTCCTGAAGCCTCAGCACTGCCAG(+ATATAT)GATCCACATTCCTGACCTGGATACTGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606221012_ejm1 ACTTTTCAGCCAGTATCCAGGTCAGGAATGTG(+ATATAT)GATCCTGGCAGTGCTGAGGCTTCAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606221010_e0tz TGTAGCCTTGCCCTGTCTTGGGCCTGGTGGAG(+ATATAT)GATCCTGGAGCCTCTTGGTGGTGCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606221008_2nuz AGAAAGGACAATGCACCACCAAGAGGCTCCAG(+ATATAT)GATCCTCCACCAGGCCCAAGACAGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606221005_x2rd ATTTGGCAACCTAACCAGGCAGCTGCCCCTGG(+ATATAT)GATCCCTAGCTTCTCCAACCTGAGCACCAGC NGG 10 8 17 8 24 0 100 5 10