Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220344_xmi8 CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+ATATAT)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606220342_rki8 TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+ATATAT)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606220341_jfbj TGGTGGCTCACAACCATCTGTAACGGGATCTG(+ATATAT)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10
    240606220339_qndu AGTAGGCTAGTCTGGCTGGCCAGCACCACAGG(+ATATAT)GATCCTGTCTCTACCCTCCAGCATGGGCATC NGG 10 8 17 8 24 0 100 5 10
    240606220337_109o CTTGTGATGCCCATGCTGGAGGGTAGAGACAG(+ATATAT)GATCCCTGTGGTGCTGGCCAGCCAGACTAGC NGG 10 8 17 8 24 0 100 5 10
    240606220335_hxrf CCTGGCAGACCCTAAGGGTTTTAGGCAGCTGG(+ATATAT)GATCCAGTGGGGAGGGCTGGCAAAGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606220333_dukt TAATTCCTTTCCTTTGCCAGCCCTCCCCACTG(+ATATAT)GATCCCAGCTGCCTAAAACCCTTAGGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606220332_w1d7 AGGACATCCACATACATAGTGCTCAATTCTTG(+ATATAT)GTACCAGAAGCAACCTTATTCTACTTACTGA NGG 10 8 17 8 24 0 100 5 10
    240606220330_spdy ATTTGTCAGTAAGTAGAATAAGGTTGCTTCTG(+ATATAT)GTACCAAGAATTGAGCACTATGTATGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606220328_romg CTGTCAGGGTTCTCTTTCCAGTGCTTCCTCGG(+ATATAT)GTACCTGATGGAAGAGAATGTCCTGGAAGCC NGG 10 8 17 8 24 0 100 5 10