Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215113_ffz7 CAACACTGCACCACTGAACTGTGTCCCCCAGG(+ATATAT)GTACCATCACAGAGAATGGACAAAAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606215112_djoj GTGGGCTCATTTTTTGTCCATTCTCTGTGATG(+ATATAT)GTACCCTGGGGGACACAGTTCAGTGGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606215111_xy7s TGCTAGTTGCTCTGAAAGATTGGGACCTCTTG(+ATATAT)GATCCCCCCCCCCATGGACTCTGATTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606215108_khli ATAGACAGACAATCAGAGTCCATGGGGGGGGG(+ATATAT)GATCCAAGAGGTCCCAATCTTTCAGAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606215106_ydcm AACCCCCGCCAGCCCAGTAGGGTAGCCAGGAG(+ATATAT)GATCCCTGAGTCCAACCCGTAAACACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606215105_z8oc TGTGGCTCTAGTGTTTACGGGTTGGACTCAGG(+ATATAT)GATCCTCCTGGCTACCCTACTGGGCTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240606215103_fxy1 GCTATAGAGGGCAAACCTGTTTGTATTCATGG(+ATATAT)GATCCAACAAAATACATGAAAGGATGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606215101_v6v3 CATATTGGCTCATCCTTTCATGTATTTTGTTG(+ATATAT)GATCCCATGAATACAAACAGGTTTGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606215100_0g79 TCACTGAGCTCTGTTCTCTGTCTGCAGCACAG(+ATATAT)GTACCAAAGAGGGGTGCAATGGAGGGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606215059_nir6 TCAGAGCTAGCCCTCCATTGCACCCCTCTTTG(+ATATAT)GTACCTGTGCTGCAGACAGAGAACAGAGCTC NGG 10 8 17 8 24 0 100 5 10