Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215007_9pvj TGAAACACAGTTACCATTTTGTGTGGATTTAG(+ATATAT)GTACCCCAAAATGTTTTTTTAAGTGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606215005_uzuv CCTATTTGTTTCACTTAAAAAAACATTTTGGG(+ATATAT)GTACCTAAATCCACACAAAATGGTAACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606215003_qblh GTGCATGTGTGTGTGTCCCACTGGTTCTCAGG(+ATATAT)GATCCAATTCCAACCACTAACAAAGACACCT NGG 10 8 17 8 24 0 100 5 10
    240606215001_jlw6 ATATTAGGTGTCTTTGTTAGTGGTTGGAATTG(+ATATAT)GATCCCTGAGAACCAGTGGGACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606214959_668q TGGCTTACAACTGTCTATACCTCAGTTCCAGG(+ATATAT)GATCCAGTGCCACCTTATGGTTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606214957_cvde TGCATGCCTGTGGGAACCATAAGGTGGCACTG(+ATATAT)GATCCCTGGAACTGAGGTATAGACAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606214955_vdhs CTATTTAACTTTCCCTTAAATTTTAAGATTTG(+ATATAT)GATCCCATGTATGTCATCATGTCCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606214953_mgil AAATATTCTAGGGGACATGATGACATACATGG(+ATATAT)GATCCAAATCTTAAAATTTAAGGGAAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606214952_441o GTAAAGAGCATGTAAATACCACAGGAAGACAG(+ATATAT)GATCCCTGGAGACATTGAAGGAACAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606214949_qyug CCTCCCTCTCTTGTTCCTTCAATGTCTCCAGG(+ATATAT)GATCCTGTCTTCCTGTGGTATTTACATGCTC NGG 10 8 17 8 24 0 100 5 10