Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214948_360r TCGTAGTCGCTAAAATTCACTCAATTCACATG(+ATATAT)GATCCCAGCTGTGTGTTGTAAGGCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606214946_azqf TCCACTGACTCAGCCTTACAACACACAGCTGG(+ATATAT)GATCCATGTGAATTGAGTGAATTTTAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240606214945_nv4j TCCACTTTGAACGTTTTCAGAAATCCCATAAG(+ATATAT)GATCCTGGAGTGATAGATGATTTCTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606214943_n2ty TTCACCAAGACAGAAATCATCTATCACTCCAG(+ATATAT)GATCCTTATGGGATTTCTGAAAACGTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606214942_ykme TGTGTATGTCTGTGTACCAGAAGACGACATTG(+ATATAT)GATCCCATAGATCTGTTGTATGGATGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606214940_0ero GACTTACAGCCATCCATACAACAGATCTATGG(+ATATAT)GATCCAATGTCGTCTTCTGGTACACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606214938_qtoc GTAAAGTAGTAGCTAACCCCTCCAAGGCCCAG(+ATATAT)GATCCTAGTTCCAGTGTTACAGAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606214937_mlmg AAAAGGAAACATTTCTGTAACACTGGAACTAG(+ATATAT)GATCCTGGGCCTTGGAGGGGTTAGCTACTAC NGG 10 8 17 8 24 0 100 5 10
    240606214935_3xii CACTTTACTGTTTCAATGGTAATAATGCACCG(+ATATAT)GTACCTCAATTCAACTGCTACAAATAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606214934_svae TTTTGACATTTATTTGTAGCAGTTGAATTGAG(+ATATAT)GTACCGGTGCATTATTACCATTGAAACAGTA NGG 10 8 17 8 24 0 100 5 10