Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212149_x6j7 CTGTAATTTCTAATTATGAGCTCACCTATTTG(+ATATAT)GTACCGAGCATGCCAATTTTAAAGAGACCCG NGG 10 8 17 8 24 0 100 5 10
    240606212147_06uv ATCCAGAGCAGTGATGATGAGCAGGTAGACGG(+ATATAT)GTACCAGCCGGCGGGCTTCTGGATAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606212145_8y95 ACAGGGACCCCTATCCAGAAGCCCGCCGGCTG(+ATATAT)GTACCCGTCTACCTGCTCATCATCACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606212144_e7cw CTAGCATGAGACTTAGAAGCTCCTGATCCAAG(+ATATAT)GTACCAGATGTCATAGTTACCTAAGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606212142_dzx1 AAATTCCTTCTCTTAGGTAACTATGACATCTG(+ATATAT)GTACCTTGGATCAGGAGCTTCTAAGTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606212140_na2m TCCCAAACTTAGGGCTTCCTCCATGTGCAAGG(+ATATAT)GTACCGTCACTGAGACACATCGCTAGCACAA NGG 10 8 17 8 24 0 100 5 10
    240606212139_26cu TAAGCTTGTGCTAGCGATGTGTCTCAGTGACG(+ATATAT)GTACCCTTGCACATGGAGGAAGCCCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606212137_cc8w CTCGAATCCAAAACTGTATAGACGTTCCAAAG(+ATATAT)GTACCTGTTTTCTAAGCTCTAATCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606212136_pkta GAAAGAGAAGAGGATTAGAGCTTAGAAAACAG(+ATATAT)GTACCTTTGGAACGTCTATACAGTTTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606212133_cos9 ACAAGACAACACCACCACGGAAGCAGAGGGGG(+ATATAT)GATCCATGCACAGCCTTTTCAGGGTCTCTTG NGG 10 8 17 8 24 0 100 5 10