Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212113_fj1j TCTATTATTTTAGCTGGGGATACGTTTGGATG(+ATATAT)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606212110_z7ez AAAATGTCCTTGATAATAAATGGTATGGCCCG(+ATATAT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606212109_05cv ACAGATAGCTCCCCTGGATCTTATCAAAATAG(+ATATAT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606212107_ni1o TAGTCCACTGCCAAGTGTGGCCCTAGGCATGG(+ATATAT)GATCCCGGGGGCTCCAGGAAAATGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240606212104_kob4 ATGACACAATAGCATTTTCCTGGAGCCCCCGG(+ATATAT)GATCCCATGCCTAGGGCCACACTTGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606212102_gt4j AAAAAAACAAACAAAGCAAAAACAAACAAAAG(+ATATAT)GATCCCCTAGAAACAGTAAGGTAAATCTATT NGG 10 8 17 8 24 0 100 5 10
    240606212100_xowy AGAAAAATAGATTTACCTTACTGTTTCTAGGG(+ATATAT)GATCCTTTTGTTTGTTTTTGCTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606212059_q1nl TTAGGTAAAATTATAGGAAAAATTATTGAAAG(+ATATAT)GTACCCTTCAATGGACAGTGTCATCTATTTC NGG 10 8 17 8 24 0 100 5 10
    240606212056_z3mo TACTAGAAATAGATGACACTGTCCATTGAAGG(+ATATAT)GTACCTTTCAATAATTTTTCCTATAATTTTA NGG 10 8 17 8 24 0 100 5 10
    240606212055_zmkq TCTAGAAAGCATATTTAAAATCGTTGGTATCG(+ATATAT)GTACCAGGAGAAGCCAGGCTAAGAAATAAAG NGG 10 8 17 8 24 0 100 5 10