Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212019_bzro GCAGTCACCCAAGACACCCTCTATCTGGGGTG(+ATATAT)GTACCTGGTGTAGTTTGTAGCTCAAACCCAA NGG 10 8 17 8 24 0 100 5 10
    240606212018_xj7v CTGTGTGTATGTGCATGCAAACTCAGAGGAAG(+ATATAT)GTACCAGGCTCCCTGGGACTAGAGTTATGGA NGG 10 8 17 8 24 0 100 5 10
    240606212016_gb2o CGCCATCCATAACTCTAGTCCCAGGGAGCCTG(+ATATAT)GTACCTTCCTCTGAGTTTGCATGCACATACA NGG 10 8 17 8 24 0 100 5 10
    240606212014_nbp3 TGACCCAGCGCTGTGCAAGGGTTGGTTCTGGG(+ATATAT)GATCCAAGAACGTGTATTTGCTGTTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606212012_64pr CGCCCAGAGATAACAGCAAATACACGTTCTTG(+ATATAT)GATCCCCAGAACCAACCCTTGCACAGCGCTG NGG 10 8 17 8 24 0 100 5 10
    240606212011_h2pj ATTCAGTAACTTAAATATTGCCAGTGACTGAG(+ATATAT)GATCCCCTCCAGTCATGGTTCTGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240606212008_v3yv CTCAAAGAATATACAGAACCATGACTGGAGGG(+ATATAT)GATCCTCAGTCACTGGCAATATTTAAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606212006_q8pf ATAGATACTTTGCATTTATGGCTTGAATGAAG(+ATATAT)GTACCAATTTTGATCTGGAGTTCTAAATGTG NGG 10 8 17 8 24 0 100 5 10
    240606212005_aavp ATTTTCACATTTAGAACTCCAGATCAAAATTG(+ATATAT)GTACCTTCATTCAAGCCATAAATGCAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606212004_voau GGCGGCTGTGGTCCAGCAGGATCTACATGTAG(+ATATAT)GTACCTACTCTTTGGTGCAATAGTGTTACGA NGG 10 8 17 8 24 0 100 5 10