Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211947_uxm2 CAGACCGTGAGGTCCTAGGCACCCAGGAAACG(+ATATAT)GATCCAGAGCCCAGGCAAGTGTCTTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240606211946_mnpt CCCCAGCAATCCAGTCATCTCCTGTCAGGTTG(+ATATAT)GATCCAGGAGAAGTGAGGTCAAAACTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606211944_2k05 TTGACTCTTCAGTTTTGACCTCACTTCTCCTG(+ATATAT)GATCCAACCTGACAGGAGATGACTGGATTGC NGG 10 8 17 8 24 0 100 5 10
    240606211942_q8le TCCTCATCATGGCTTCCCAGACCACCTCACTG(+ATATAT)GTACCTTTTGCTATGTTAGCTTGTTATGAGT NGG 10 8 17 8 24 0 100 5 10
    240606211941_lly2 AGCTAACTCATAACAAGCTAACATAGCAAAAG(+ATATAT)GTACCAGTGAGGTGGTCTGGGAAGCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240606211938_e03q CACAAATGACTAAGCACCTGGCAGGGGCTGGG(+ATATAT)GTACCTTTCAGCAGAGCCTTTGCTGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606211937_znnk TGCTTCATGCTCAGCAAAGGCTCTGCTGAAAG(+ATATAT)GTACCCCAGCCCCTGCCAGGTGCTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606211935_w49y CCTGGAGCGCTCTCTCCTGGAGGCAGCGAGGG(+ATATAT)GTACCACCTCTGCAAATAGACCAGGAAGCGG NGG 10 8 17 8 24 0 100 5 10
    240606211934_3a0y TGGCACCGCTTCCTGGTCTATTTGCAGAGGTG(+ATATAT)GTACCCCTCGCTGCCTCCAGGAGAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240606211932_qocx ATGGACTTCCTTGTCTAAAGGCCACCTCGAGG(+ATATAT)GTACCACCTCAACATGCCAGGGTACTGTTTT NGG 10 8 17 8 24 0 100 5 10