Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211857_mjis GATGTATATGAAAATCTGGCTTTTGAAGACTG(+ATATAT)GATCCACGACCACATACATCTAGAAGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606211854_096y ATGTATCTTGTTACCTACTCATCCAGCCGTGG(+ATATAT)GTACCTCGGTTGTTCCCACCCTGGAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606211852_v7ru TAGTCAGGAGCTCCAGGGTGGGAACAACCGAG(+ATATAT)GTACCCACGGCTGGATGAGTAGGTAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240606211851_ycfn TAAAGGCAGCTGAGCTGCAGGAGCTCTGACAG(+ATATAT)GATCCGCACGGCATTCCCAGCTGACCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606211850_ql2a TGCAGTCTCCGGTCAGCTGGGAATGCCGTGCG(+ATATAT)GATCCTGTCAGAGCTCCTGCAGCTCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606211848_179z CATCACAGCAATAGTAACCCTAATGAAGACAG(+ATATAT)GATCCCAGCACCCATGCTGGGTAGCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240606211847_0o4b TGCTGTTGTAAGCTACCCAGCATGGGTGCTGG(+ATATAT)GATCCTGTCTTCATTAGGGTTACTATTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606211845_dm14 TAAGAGAGTTTATTCGGGTTCTGCACTCAGAG(+ATATAT)GTACCTACAACAGACTACAGACAAAATCAAT NGG 10 8 17 8 24 0 100 5 10
    240606211844_bmsl TCCTAATTGATTTTGTCTGTAGTCTGTTGTAG(+ATATAT)GTACCTCTGAGTGCAGAACCCGAATAAACTC NGG 10 8 17 8 24 0 100 5 10
    240606211841_bv8g GACTTTGTACTCTGGCTGTATCCCATCAGAAG(+ATATAT)GTACCATTGTAAGACTCTGGTGAGCCATAGC NGG 10 8 17 8 24 0 100 5 10