Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211810_mgpx CTCACCCTAGCCTCAGAGCACAGTGGCTGCAG(+ATATAT)GTACCAACTGCGTTGTATCATCTGGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606211808_8qfr TCTCATTGCTTCAGCCTCTTCTCTGCTGGGTG(+ATATAT)GATCCTGTTTCTGACTGTGGCTGTGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606211807_lw4i AAAGCAGAAGACACAGCCACAGTCAGAAACAG(+ATATAT)GATCCACCCAGCAGAGAAGAGGCTGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606211805_8q7y CATGAGCCGGGCTGGAAACTTCGTTGATGTGG(+ATATAT)GATCCAGGCTCCATATGTAGTCCTGGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240606211803_9zr9 CTGCTATCCTCCAGGACTACATATGGAGCCTG(+ATATAT)GATCCCACATCAACGAAGTTTCCAGCCCGGC NGG 10 8 17 8 24 0 100 5 10
    240606211801_ay6i CGAGGAGGTCGTGCTAGATGGCCTGTTGGTAG(+ATATAT)GATCCATGCTGGGGCTGGGCCTCTTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240606211800_hj3a GCTGTGAGTACAAGAGGCCCAGCCCCAGCATG(+ATATAT)GATCCTACCAACAGGCCATCTAGCACGACCT NGG 10 8 17 8 24 0 100 5 10
    240606211758_5kat AGGTGATGTAAGGCCAGCAAGTTGGTTCCTGG(+ATATAT)GATCCCCAGGTCTGGCATAGTTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606211755_txz9 AATGCCAACTATCCAACTATGCCAGACCTGGG(+ATATAT)GATCCCAGGAACCAACTTGCTGGCCTTACAT NGG 10 8 17 8 24 0 100 5 10
    240606211754_o9jp GATGAAGGGGATCCTTCCACGCACGCACGAAG(+ATATAT)GTACCCTCTGTCTATCCTGGGACCTGACCCC NGG 10 8 17 8 24 0 100 5 10