Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211826_kv32 GGAGGTAGGAACTGATGTAGGGGCCATGGAGG(+ATATAT)GATCCTGCTTACTGGCTTGCTTCTCATGACT NGG 10 8 17 8 24 0 100 5 10
    240606211825_yp48 CAAAAGTCAATCTCCAACCGTCCAATGTCTGG(+ATATAT)GATCCAACTTACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606211824_3bns GCCCTTGGAGGAGCCCAGAAGATCGTAAGTTG(+ATATAT)GATCCCAGACATTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10
    240606211822_jgl2 AGGCAGCCTTGCTTACCGTATGTGAGGTCAGG(+ATATAT)GTACCGGGTGCTGGGGATTGCTTGTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606211819_ricf GTGAGTGGGCCACAAGCAATCCCCAGCACCCG(+ATATAT)GTACCCTGACCTCACATACGGTAAGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606211818_is3q ATAGCAGGTCCTTAAGTGGGAATGAGCTCAGG(+ATATAT)GATCCTCAGCCAGCCATGCAGGCAGGGCGCA NGG 10 8 17 8 24 0 100 5 10
    240606211816_wqnh GTCGTTGCGCCCTGCCTGCATGGCTGGCTGAG(+ATATAT)GATCCCTGAGCTCATTCCCACTTAAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240606211815_9tyf ACAGCATAGCAGAAGTTTTTAATAGGTGACTG(+ATATAT)GTACCACAGTAAGAAGGAATCATTTCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606211813_png8 ACGGGCAGGCGAAATGATTCCTTCTTACTGTG(+ATATAT)GTACCAGTCACCTATTAAAAACTTCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606211812_x9vq TCAGCTGTGCTCCAGATGATACAACGCAGTTG(+ATATAT)GTACCTGCAGCCACTGTGCTCTGAGGCTAGG NGG 10 8 17 8 24 0 100 5 10