Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211659_w5zi TGGAGTTACAGACAGTTGGGAGCCATCATCTG(+ATATAT)GATCCTGGAGATTGAACCTCCATCTTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240606211657_8076 ACACACACACACCACACGCAAAGCCATGGGAG(+ATATAT)GATCCCAGAGGCCTTGTTGAGAATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240606211655_u9ry CTCATGAATCCAATTCTCAACAAGGCCTCTGG(+ATATAT)GATCCTCCCATGGCTTTGCGTGTGGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606211654_jz3b ACAAGGACACTGGGAGGTGTGTGAACGCCATG(+ATATAT)GTACCGGCGGCTGCTTGAGGCGTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606211651_7o38 GAGTCACAGGAAAACGCCTCAAGCAGCCGCCG(+ATATAT)GTACCATGGCGTTCACACACCTCCCAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606211649_2l22 GGTGCTCCCAGGGCACTCACCACAGCCACCAG(+ATATAT)GATCCTCCAGAGGCAAGCAGCATCTCAGATT NGG 10 8 17 8 24 0 100 5 10
    240606211648_ottg CTTGTAATCTGAGATGCTGCTTGCCTCTGGAG(+ATATAT)GATCCTGGTGGCTGTGGTGAGTGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606211646_6gwz AAATCACGTCTCACAGCAGGCACCACTGTGTG(+ATATAT)GATCCAAGTCCCCCACCTTACCCTGCACCAG NGG 10 8 17 8 24 0 100 5 10
    240606211645_o3sr CGCCCCTGGTGCAGGGTAAGGTGGGGGACTTG(+ATATAT)GATCCACACAGTGGTGCCTGCTGTGAGACGT NGG 10 8 17 8 24 0 100 5 10
    240606211644_ggo1 ACCACAAATTTATTGTTTATAGCTGAGATCAG(+ATATAT)GATCCGAGCAATCCCGTTTCAGCTGCCCAGT NGG 10 8 17 8 24 0 100 5 10