Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211554_9l34 AAACATGTGGACACGCCATTTCTGAGACACAG(+ATATAT)GTACCTCCTTCACAGCTACTGCCTGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606211553_pand ACCAAGGGGGGAAATCCCAGCAGATAATTTAG(+ATATAT)GATCCCCTTCTAAACACATGAGGGCCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606211551_klar TGCACATACAGGCCCTCATGTGTTTAGAAGGG(+ATATAT)GATCCTAAATTATCTGCTGGGATTTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606211550_p5hu AATGTTTAGAGAACAATTAGGTCAATTTCAGG(+ATATAT)GATCCACTTGGTGTATGTGTGGACCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240606211548_9u1g ACCTATCTGTAGGTCCACACATACACCAAGTG(+ATATAT)GATCCCTGAAATTGACCTAATTGTTCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240606211547_9qd6 ATGCCTACTTGATTTCTATTAAAAAGAAAAAG(+ATATAT)GTACCAGGTAATTCTATTTGGGAAACGAATG NGG 10 8 17 8 24 0 100 5 10
    240606211545_4abx TAGTGCATTCGTTTCCCAAATAGAATTACCTG(+ATATAT)GTACCTTTTTCTTTTTAATAGAAATCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606211543_zqqp GTATTTACGGATAGTTGTAAGCCACTGTGTGG(+ATATAT)GTACCGGGAATTGAACCCAGGTCCTCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606211540_2wu7 AGCACTAGCAGAGGACCTGGGTTCAATTCCCG(+ATATAT)GTACCCACACAGTGGCTTACAACTATCCGTA NGG 10 8 17 8 24 0 100 5 10
    240606211539_1cve TAATGTCCTACTCCTGGCTTGCAATACATGTG(+ATATAT)GATCCTCTTCAGTTATGAATGGAAAATGCCA NGG 10 8 17 8 24 0 100 5 10