Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211442_se8m TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ATATAT)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240606211441_4ltc CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+ATATAT)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606211440_01hm CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ATATAT)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606211438_l34i ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ATATAT)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606211434_y62o TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ATATAT)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240606182853_uq7i GCCAGGGTGAGGGGAAGCCGGCCAGGCTGGCG(+AAAAAA)GATCCGACCCGGCGAGGCGGTCGCGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606182851_j3sk CCGATGGAAAACGCGACCGCCTCGCCGGGTCG(+AAAAAA)GATCCGCCAGCCTGGCCGGCTTCCCCTCACC NGG 10 8 17 8 24 0 100 5 10
    240606182850_tj4c TGAAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AAAAAA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240606182848_5zye ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AAAAAA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606182846_36rs ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AAAAAA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10