Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212648_b4bq GAGGCCTGGCCTGTGCTTCCCTGAGCAGAGAG(+ATATAT)GATCCCAGTCCTCCTGGGCAGGCAGCCCCGC NGG 10 8 17 8 24 0 100 5 10
    240606212647_9jdt AGGCAAGGGAGGAGCAATCATTTTCCGCAATG(+ATATAT)GTACCAATGGATGGGGAGGTACTGGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606212645_w4ln CCCACCCCTCACCAGTACCTCCCCATCCATTG(+ATATAT)GTACCATTGCGGAAAATGATTGCTCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606212643_6kh0 AGTGCAATCTGAAATGACCTGGACAAGGAATG(+ATATAT)GTACCCCATTGGGTTGAAAAATTCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240606212641_718p TCCTGGCTATGTGAATTTTTCAACCCAATGGG(+ATATAT)GTACCATTCCTTGTCCAGGTCATTTCAGATT NGG 10 8 17 8 24 0 100 5 10
    240606212637_dfrh CCACAGACCCATTGGGGCCAGGTCTAGCTCAG(+ATATAT)GTACCTGAGCAAATGTTCCTTTAGAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606212636_7psm CCCTGGTGGACTCTAAAGGAACATTTGCTCAG(+ATATAT)GTACCTGAGCTAGACCTGGCCCCAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606212633_cx6c ACTTGTTAAGAGGCAAGGCCACCTTTCACCAG(+ATATAT)GTACCCTGGCCAGGCACCTGGGAAGACTCTT NGG 10 8 17 8 24 0 100 5 10
    240606212631_gexc GCCAGAAGAGTCTTCCCAGGTGCCTGGCCAGG(+ATATAT)GTACCTGGTGAAAGGTGGCCTTGCCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606212629_s66d TCCTGGGCAGCCTGGAGGGCTGGCATGCCTGG(+ATATAT)GATCCTCGGCCACCCAGGATCTTGGAGACAA NGG 10 8 17 8 24 0 100 5 10