Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606211627_ug8s AGGGCTCTGGTGGCATGCTCTCAGCGTCAGTG(+ATATAT)GTACCCACCAGCTAACTATCAGCAAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606211625_i428 TTCCACAGTGGCATGGCCACCCAGCCTCGGGG(+ATATAT)GATCCTGGGCTCGCTGCACAGCACCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606211623_zs9h GAAGAGATCTGGGTGCTGTGCAGCGAGCCCAG(+ATATAT)GATCCCCCGAGGCTGGGTGGCCATGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606211621_hl9e AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+ATATAT)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606211620_vnzm GTCTGTTACACCTGGACACTGGTACAGGAGAG(+ATATAT)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606211619_d8yx TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+ATATAT)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240606211617_obnd TATTGAGATCTAATGACAATGAATGATATGTG(+ATATAT)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606211616_e2x7 GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+ATATAT)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606211615_4t87 GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+ATATAT)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606211613_2ezl AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+ATATAT)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10