Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212820_frcz TATGGCTTTGCATGTAAAACACTTTTGATTGG(+ATATAT)GATCCACAGCAGTGTTCTTTTCACCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606212816_nx3d CCAGGCCAGCACAGTGCTTCCTGGTAGGGTGG(+ATATAT)GTACCAGCCATACTGAGAATCGGGCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606212815_nv70 GAGCAGGCCTGGCCCGATTCTCAGTATGGCTG(+ATATAT)GTACCCACCCTACCAGGAAGCACTGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606212813_32he TCTCTTCAGGGGCCGCTTTCCTGGTGTCAGAG(+ATATAT)GTACCCATGGTGGAGCAGAGCCACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606212811_nnr8 TCTGGGAGGAAGGTGGCTCTGCTCCACCATGG(+ATATAT)GTACCTCTGACACCAGGAAAGCGGCCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606212810_2q8n TCTCTAGCACCTGGCTCCTGTGGCTCTCTATG(+ATATAT)GTACCTGGAATCCCCAAGGTGTTTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606212807_nn8n GAGAAAGGAGTTAAACACCTTGGGGATTCCAG(+ATATAT)GTACCATAGAGAGCCACAGGAGCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606212805_chde ATCTTACAGTCAGCAATCTGGGGGTGGGGAGG(+ATATAT)GATCCCTCTCTAGCACCTGGCTCCTGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606212803_8guh TAGAGAGCCACAGGAGCCAGGTGCTAGAGAGG(+ATATAT)GATCCCTCCCCACCCCCAGATTGCTGACTGT NGG 10 8 17 8 24 0 100 5 10
    240606212801_aziy GGCAAAGACTGAGCTCAGGCACCAGCCTCCCG(+ATATAT)GTACCACGCTGGAGGTGGATCCCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10