Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213143_mbjm ACTACAACAACAGTTTAGTAAGAGAAACATGG(+ATATAT)GTACCTATAGAGTCCTACCATTCATTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606213141_lgyw TAAATCCTGTCTACAAAAATTATGTCATACAG(+ATATAT)GTACCATTTGTTCTTTCTCTTTCCAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606213138_kudv AATGGACTCTTTGGAAAGAGAAAGAACAAATG(+ATATAT)GTACCTGTATGACATAATTTTTGTAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606213137_6fpl GAGACGAGTTTCTGAAAATATACTGCACAATG(+ATATAT)GATCCTAGGAGAGCCCAATCAAAGGCCTACT NGG 10 8 17 8 24 0 100 5 10
    240606213135_obb9 AAGAGAGTAGGCCTTTGATTGGGCTCTCCTAG(+ATATAT)GATCCATTGTGCAGTATATTTTCAGAAACTC NGG 10 8 17 8 24 0 100 5 10
    240606213133_5r0n TGGTGTACCAGGCACTATTATTCTTACAGAGG(+ATATAT)GTACCATGAAAGTTAGATCCAGGTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606213132_fpxp GGAGCCAAAGGCACCTGGATCTAACTTTCATG(+ATATAT)GTACCCTCTGTAAGAATAATAGTGCCTGGTA NGG 10 8 17 8 24 0 100 5 10
    240606213131_3uaz CACTGCAAGGTAGTATTTACCTGATCACAAAG(+ATATAT)GATCCTTTGAGGCTCTACCACATATTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606213129_u1da CTAGAATTTGAATATGTGGTAGAGCCTCAAAG(+ATATAT)GATCCTTTGTGATCAGGTAAATACTACCTTG NGG 10 8 17 8 24 0 100 5 10
    240606213128_dizf GGCTTAGTTTTTATTTAATAAGATATTTAATG(+ATATAT)GATCCATTTTTATCAAGGTCAGAAAACATTT NGG 10 8 17 8 24 0 100 5 10