Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182712_ghbj ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AAAAAA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606182711_jiby TGATCTTGTAATAGTTCTATCTTTAGATCTTG(+AAAAAA)GATCCAACTTGCTGCATCTTTCTGTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606182710_07wm AGCTACTACAGACAGAAAGATGCAGCAAGTTG(+AAAAAA)GATCCAAGATCTAAAGATAGAACTATTACAA NGG 10 8 17 8 24 0 100 5 10
    240606182707_6gb5 CTGCAGAGACTTGATGTGCCTGGTGGGAGGGG(+AAAAAA)GTACCACCCTCTCAGAGAAAAAGGGGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606182706_v187 ATAACTCCCTCCCCTTTTTCTCTGAGAGGGTG(+AAAAAA)GTACCCCCTCCCACCAGGCACATCAAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606182704_mkgz GTGATCAAGGCAGAGAAGGCTCATTGTGGGTG(+AAAAAA)GTACCATCCCTGGGCTGGTAGTTTTGGGATC NGG 10 8 17 8 24 0 100 5 10
    240606182702_3rc1 TTACAGATCCCAAAACTACCAGCCCAGGGATG(+AAAAAA)GTACCACCCACAATGAGCCTTCTCTGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606182701_jj5v ACTCTAGAGAACCCTGACTAATACATAAGTTG(+AAAAAA)GTACCAGGGACTGAGGTATTGCTGTGATAGG NGG 10 8 17 8 24 0 100 5 10
    240606182658_93ft TCAGGCCTATCACAGCAATACCTCAGTCCCTG(+AAAAAA)GTACCAACTTATGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606182657_zhwh GAGCCTTCAGTAGTAATTGGGAAAAGGTAATG(+AAAAAA)GATCCATTCATATGCGAAGTAAACATGACAT NGG 10 8 17 8 24 0 100 5 10