Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181410_gu7e ATCTTTTATATACTCTGCATAACAGACATGAG(+AAAAAA)GTACCAGTGAAGTTATGCAGACCAGTACCTG NGG 10 8 17 8 24 0 100 5 10
    240606181409_ngz8 CTTGAAAACTGAATCTGTGCACCTTTAGACTG(+AAAAAA)GATCCATCAATGTGTATTTCTGAACAATTCC NGG 10 8 17 8 24 0 100 5 10
    240606181408_4ndx GAATGGGAATTGTTCAGAAATACACATTGATG(+AAAAAA)GATCCAGTCTAAAGGTGCACAGATTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606181406_480e ATCAAACAAACAGCAAGGAACATCCTGGGTTG(+AAAAAA)GATCCCTTAGACCTCCAAATGCCTTGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606181404_xawp TATGGAACCACAAGGCATTTGGAGGTCTAAGG(+AAAAAA)GATCCAACCCAGGATGTTCCTTGCTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606181403_qun4 AAGCAAAGAATATACCTTCTCAGCACCTTATG(+AAAAAA)GTACCTTTTCCAAAATTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240606181402_d4iy CGTGACCAATTATATGGTCAATTTTGGAAAAG(+AAAAAA)GTACCATAAGGTGCTGAGAAGGTATATTCTT NGG 10 8 17 8 24 0 100 5 10
    240606181359_xdfc GGATGGACCATCTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606181358_6gz6 GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606181357_2d2r GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+AAAAAA)GATCCCCGGATATGTCAATCTCTAGTTGGTC NGG 10 8 17 8 24 0 100 5 10