Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181515_owu7 ATTACCCCCAGAAGAGTATAGAACCATGTCAG(+AAAAAA)GATCCACACTCTCCCCTTCTGACCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606181514_4y9y GTGACCATTTTTTACACAACCATACTGGAAAG(+AAAAAA)GATCCAGTACCTTTTTTTTTTTAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240606181513_30uy TCTGAAATCACACTAAAAAAAAAAAGGTACTG(+AAAAAA)GATCCTTTCCAGTATGGTTGTGTAAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606181511_9dw0 TCTTTTCATCAAAAACCTTATCATTTCCTATG(+AAAAAA)GTACCATTGTCTGTCTCCAGAGTGTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606181510_qhfr CAGATTTTGACACACTCTGGAGACAGACAATG(+AAAAAA)GTACCATAGGAAATGATAAGGTTTTTGATGA NGG 10 8 17 8 24 0 100 5 10
    240606181508_uysl ACTGTGTAGCCTAGGTTGATATCAAATCAATG(+AAAAAA)GTACCCTCAGCACACATACCAGTTCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606181507_1yyy TAACTGAGGCTGAACTGGTATGTGTGCTGAGG(+AAAAAA)GTACCATTGATTTGATATCAACCTAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240606181506_o490 TAGACAAGCCACTTGTTGTATCTGAGTTTTAG(+AAAAAA)GTACCAGAGGGGAAATACTGCCTGATCGATT NGG 10 8 17 8 24 0 100 5 10
    240606181504_0hxi ATAAAAATCGATCAGGCAGTATTTCCCCTCTG(+AAAAAA)GTACCTAAAACTCAGATACAACAAGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606181501_w9j8 GCTGTAATTCCAGGCTTCTTGGAAAGCTCCGG(+AAAAAA)GATCCGTTTTAGGAAAGCACCGAGAGGTAAG NGG 10 8 17 8 24 0 100 5 10