Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181546_sgob TTTAGGACCTGTAGCTTGAGACGCTATTCTAG(+AAAAAA)GATCCTTATTTTAACTTGAAAGAGATTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606181545_gqvn ACTGTAAAGGAAGAAAGAGGGAAAGGAGGTAG(+AAAAAA)GTACCAAGGTAGGCACTGCCAAGCTCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606181543_k3xg GCTCACTTGAGAGCTTGGCAGTGCCTACCTTG(+AAAAAA)GTACCTACCTCCTTTCCCTCTTTCTTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606181541_vzwa GCTATTTCTTCAATCTTACCAACTCAGAGGTG(+AAAAAA)GTACCTACAAATGTTAACTCTCATTAATCGT NGG 10 8 17 8 24 0 100 5 10
    240606181540_xi2f GATATACGATTAATGAGAGTTAACATTTGTAG(+AAAAAA)GTACCACCTCTGAGTTGGTAAGATTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606181539_77lv TGTTTCAAAATGCCTTTCCCTTTCCCACTCTG(+AAAAAA)GATCCCAGCTAGGTGATAATATGACACCCCC NGG 10 8 17 8 24 0 100 5 10
    240606181537_a6jg TTTTGGGGGGTGTCATATTATCACCTAGCTGG(+AAAAAA)GATCCAGAGTGGGAAAGGGAAAGGCATTTTG NGG 10 8 17 8 24 0 100 5 10
    240606181535_6kgq GCTAACAATGGCCCGTAACTCCAGTTCTGGGG(+AAAAAA)GATCCGACACCCTCTTCTGGCCTCTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240606181534_x5g9 CATGTGCTCATAGAGGCCAGAAGAGGGTGTCG(+AAAAAA)GATCCCCCAGAACTGGAGTTACGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240606181532_sz3q GAAACAACCCAAGCTCCTCCTCCGTCCCTCTG(+AAAAAA)GTACCAGATCTAACCAGGCATCCAAAGGCCT NGG 10 8 17 8 24 0 100 5 10