Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181653_qxbj TCTACAAAGAGTCACCCGAACTTCTTGGGTAG(+AAAAAA)GTACCTCCACTTACCAATTAGTCTCCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606181651_ln97 GGGGTACATAATAAATAGTTCTCCATGAGGAG(+AAAAAA)GATCCTAGGGTTATAAATAGGCAGTTCAACA NGG 10 8 17 8 24 0 100 5 10
    240606181648_u4rg CCCTGTGTTGAACTGCCTATTTATAACCCTAG(+AAAAAA)GATCCTCCTCATGGAGAACTATTTATTATGT NGG 10 8 17 8 24 0 100 5 10
    240606181647_gpg2 AGAAAGAGACGGTGATGCCTCTAGGAATTCTG(+AAAAAA)GATCCACGGGAGAAAAAGCAGTATCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606181644_bdq5 GCGCGCAGCCTGATACTGCTTTTTCTCCCGTG(+AAAAAA)GATCCAGAATTCCTAGAGGCATCACCGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606181643_3tcn TGCACAAGAGATGAGGAATATCAGAGAGATGG(+AAAAAA)GATCCAATTCGATATTTAAACCTTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240606181642_fnsr AGAAACCAGATAAAGGTTTAAATATCGAATTG(+AAAAAA)GATCCCATCTCTCTGATATTCCTCATCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606181640_z1xn GTCCTCTCCTTCCAGCTTTACATGGTTCCAAG(+AAAAAA)GATCCGATGGTCAGGTTTGCATTATCATGTG NGG 10 8 17 8 24 0 100 5 10
    240606181638_z44g TGAAGCACATGATAATGCAAACCTGACCATCG(+AAAAAA)GATCCTTGGAACCATGTAAAGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606181636_bskl GGATTCTCAGGAGCTTCTCTCCCTACCCAATG(+AAAAAA)GATCCACCCCCATCACACACATTTACATACA NGG 10 8 17 8 24 0 100 5 10