Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181739_b9l3 CCTTTTTCAAAGGAAATGCTCAAGGTACCAAG(+AAAAAA)GATCCCTTCATTGTGCCTTCAGCTTGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606181737_xzqa TACCAAAAGGTCTGTCTGACCAGAGACTGAAG(+AAAAAA)GTACCAAGGGAACTGGGCTTTTTTTCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240606181735_k7u1 CCCTGCGCCTGAAAAAAAGCCCAGTTCCCTTG(+AAAAAA)GTACCTTCAGTCTCTGGTCAGACAGACCTTT NGG 10 8 17 8 24 0 100 5 10
    240606181734_orf3 TGGCCTTTGGTCCCAGACGGTCCAGGTCTGAG(+AAAAAA)GATCCAATAGGGAAGCTGGAGCACTGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606181733_q3ap GTCATTTTCCCAGTGCTCCAGCTTCCCTATTG(+AAAAAA)GATCCTCAGACCTGGACCGTCTGGGACCAAA NGG 10 8 17 8 24 0 100 5 10
    240606181731_znow TTTGGGACAGACAGAGCCAAGACTATTCAGGG(+AAAAAA)GATCCTGACCCAAGCCTGAGGAGATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606181729_380t TTCCATTTCCCATCTCCTCAGGCTTGGGTCAG(+AAAAAA)GATCCCCTGAATAGTCTTGGCTCTGTCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606181728_irbs ACATGAGCAAAGAACCTATTTCTTTCGTATGG(+AAAAAA)GTACCTGAAATGTGTTCTCATTCCTGTGATA NGG 10 8 17 8 24 0 100 5 10
    240606181727_0zty GTGGGTATCACAGGAATGAGAACACATTTCAG(+AAAAAA)GTACCCATACGAAAGAAATAGGTTCTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606181726_zm1c TGCAAGAAGAACACTGCATACTGGGCATGCAG(+AAAAAA)GTACCACCACCTGTCAGCAGTTTCTCCAGGC NGG 10 8 17 8 24 0 100 5 10