Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181809_bi03 GCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGG(+AAAAAA)GTACCCCCCTCAGTCCTCTCAGTAAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240606181806_bfl9 CCTTTTAGCTTGGAGGTGGGAGCCATGCCCAG(+AAAAAA)GATCCCTTTCCCGGGCTTTGTCCATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606181805_7ddb AGGGATTTCCCATGGACAAAGCCCGGGAAAGG(+AAAAAA)GATCCTGGGCATGGCTCCCACCTCCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606181803_xl6l TCTTTCCTCCAGCCCTTTTGGTCTCTTTCCTG(+AAAAAA)GATCCTTGTCCTCCAGACTGGCCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606181802_lzi9 ACTTTCCAGCAGAGGCCAGTCTGGAGGACAAG(+AAAAAA)GATCCAGGAAAGAGACCAAAAGGGCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606181800_ltu7 GCCTTCCTCAGCCCTGACCTACCTGGCCATAG(+AAAAAA)GATCCTACAGCCTTCTCTGTACTCCACTATC NGG 10 8 17 8 24 0 100 5 10
    240606181758_bx7a CAACTGATAGTGGAGTACAGAGAAGGCTGTAG(+AAAAAA)GATCCTATGGCCAGGTAGGTCAGGGCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606181757_57ch AAACTCAGGTCCTGATATTTGTGAAACAGGTG(+AAAAAA)GTACCACTGAGCCAGGTCCATATTCCTCATT NGG 10 8 17 8 24 0 100 5 10
    240606181756_65w2 GGGTCAATGAGGAATATGGACCTGGCTCAGTG(+AAAAAA)GTACCACCTGTTTCACAAATATCAGGACCTG NGG 10 8 17 8 24 0 100 5 10
    240606181754_bsmm ATGCAGAAAAGAGGCCATCCCAACATTTCCAG(+AAAAAA)GTACCTTGTTCTCCTAACACAGGCAGCCCAC NGG 10 8 17 8 24 0 100 5 10