Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181824_2tsa GAAGAAGCAGGAGTTCAGAAGGACATATCCTG(+AAAAAA)GATCCTCCAGAATTCCTCTTCTGACCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606181823_bb1l GGTATGCCATATCCCACAGGGGCTATGAGTAG(+AAAAAA)GTACCAGATACCAACTCAGTAATTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240606181822_9fid TGCTACAAAATTAATTACTGAGTTGGTATCTG(+AAAAAA)GTACCTACTCATAGCCCCTGTGGGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240606181821_rgn5 TGATGGTCAGTGAGGAAGCTGGCCAGCAACTG(+AAAAAA)GATCCTCCAGACCCCTCTGCTTCAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606181819_pnru ATAGGGCCTTCCTGAAGCAGAGGGGTCTGGAG(+AAAAAA)GATCCAGTTGCTGGCCAGCTTCCTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240606181818_bojn ATGTCACCTAGGGAGGACCAAGGATAACCAAG(+AAAAAA)GTACCCTTTCTTGGTACTCCCAGGGCCAACC NGG 10 8 17 8 24 0 100 5 10
    240606181816_dcke TCTAGGGTTGGCCCTGGGAGTACCAAGAAAGG(+AAAAAA)GTACCTTGGTTATCCTTGGTCCTCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606181814_j0t9 CTTAGTCAGGGTCATAAGGTACTTTGGTGGTG(+AAAAAA)GATCCTGCTCACTGTTATATAAGTTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606181813_sosf TGAGACCTGCCAACTTATATAACAGTGAGCAG(+AAAAAA)GATCCACCACCAAAGTACCTTATGACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606181811_3g6x TCCAGAGGTTTTTACTGAGAGGACTGAGGGGG(+AAAAAA)GTACCCCAGCTGGAGGGCTGAAGGCCCAGGA NGG 10 8 17 8 24 0 100 5 10