Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181601_fgl7 CTTTGTTCTTTCATTTTGCTTCCTGGTGTTTG(+AAAAAA)GTACCCATATGAACCATTTACTCTACTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606181600_1iq7 TCCTTTTCGCTTAATGAAAGAGGTTGCTTCAG(+AAAAAA)GATCCATTGCTGCAGAGAATAATAATTTATT NGG 10 8 17 8 24 0 100 5 10
    240606181558_yt3c ACAGCAATAAATTATTATTCTCTGCAGCAATG(+AAAAAA)GATCCTGAAGCAACCTCTTTCATTAAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240606181557_x2e2 CAGTATTCATTCCATGAACATAAGAAATAATG(+AAAAAA)GTACCTAAAATTAAAAAACAAAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606181556_ezcb CAGGTTTTTTGTTTTTTGTTTTTTAATTTTAG(+AAAAAA)GTACCATTATTTCTTATGTTCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606181554_dzyo TTGCTGCCCATAGAAGCCAGAAGATGGGGTGG(+AAAAAA)GATCCCCTGGAACCGGAGTTAGCAGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240606181551_108y TTCACAACTGTCTGCTAACTCCGGTTCCAGGG(+AAAAAA)GATCCCACCCCATCTTCTGGCTTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240606181550_vezs CAGTATTCATTCCATGAACATAAGAAATAATG(+AAAAAA)GTACCTAAAATTAAAAAACAAAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606181549_l21d CAGGTTTTTTGTTTTTTGTTTTTTAATTTTAG(+AAAAAA)GTACCATTATTTCTTATGTTCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606181548_jd7s TCATTCCTGAATCTCTTTCAAGTTAAAATAAG(+AAAAAA)GATCCTAGAATAGCGTCTCAAGCTACAGGTC NGG 10 8 17 8 24 0 100 5 10