Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180923_hyr3 GGCCCAACCAGGAAGTCCAGCTAGGGAAAGAG(+AAAAAA)GATCCAAAGACAGGCAACGGTGTCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606180921_dj4p GTCAGTACTCACTCACCAGACTCGGGCAGAAG(+AAAAAA)GATCCTAAGTTCAAAACCAGCCTGGAATACG NGG 10 8 17 8 24 0 100 5 10
    240606180920_zmxt TACTACGTATTCCAGGCTGGTTTTGAACTTAG(+AAAAAA)GATCCTTCTGCCCGAGTCTGGTGAGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606180918_7u4q TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+AAAAAA)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606180916_bbj1 GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+AAAAAA)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606180914_ixm0 CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+AAAAAA)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240606180912_qu8t GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+AAAAAA)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606180911_y099 TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+AAAAAA)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606180909_2t3d CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+AAAAAA)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606180907_0ann TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+AAAAAA)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10