Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180831_363w GAGAAACATGGAGATAGGTTAGCACCACCGAG(+AAAAAA)GATCCTGGAGATATGTTAGCACTTGCACGGA NGG 10 8 17 8 24 0 100 5 10
    240606180829_myyo AGAAATCTGGGTAAAGAGGAGAGAGATATGGG(+AAAAAA)GTACCCTCCTCCCCTCACTGCTTGTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606180827_uxn5 GAGATGAGGGCACAAGCAGTGAGGGGAGGAGG(+AAAAAA)GTACCCCATATCTCTCTCCTCTTTACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606180825_l11p GTGAGGTGGGAGAAGTGTGTATACCCCTCCTG(+AAAAAA)GATCCTGAATGTTTCTGCCTTTCCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606180824_7ibp CCCTCCCCCAAAGGAAAGGCAGAAACATTCAG(+AAAAAA)GATCCAGGAGGGGTATACACACTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606180822_b0po ATCACAGACAATACAGCTTTTAACAAGCTGAG(+AAAAAA)GTACCTTTCTTTTTTTAGTGCTAATGATTCA NGG 10 8 17 8 24 0 100 5 10
    240606180821_2n0s TAGGTTGAATCATTAGCACTAAAAAAAGAAAG(+AAAAAA)GTACCTCAGCTTGTTAAAAGCTGTATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606180819_1xg5 GTTAGCACCTAGAGGCCGAATCTAACGTCCCG(+AAAAAA)GATCCCATGCCTTGACACCCACAGCCCAATC NGG 10 8 17 8 24 0 100 5 10
    240606180817_yz28 TCTAAGATTGGGCTGTGGGTGTCAAGGCATGG(+AAAAAA)GATCCGGGACGTTAGATTCGGCCTCTAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606180815_cju7 GAAGTCTGTGGAGCCAGTGAGGAAGACTCCTG(+AAAAAA)GATCCCTCCCCGTGATGGGTTAACCCCACAG NGG 10 8 17 8 24 0 100 5 10