Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180722_0aj2 ATAGCCCCTCCCTCTCTGCCCCGCCCCTCCGG(+AAAAAA)GTACCATAGAGTCCCTGTCCTCCCGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606180719_s73o GGATGAGCTCAGTTGGCGTCTGCTCGCCTTGG(+AAAAAA)GTACCAGCCTTGGAAAACGCTGTCGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606180718_ae44 AGCGTTTGGGGCGACAGCGTTTTCCAAGGCTG(+AAAAAA)GTACCCAAGGCGAGCAGACGCCAACTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606180716_rl7b ACTCCACCCACACTTGTGGGAACTCCAAAAGG(+AAAAAA)GTACCTGGCCCACAAGATTCTGGGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606180714_1n13 CCTATTTCTGACCCCAGAATCTTGTGGGCCAG(+AAAAAA)GTACCCTTTTGGAGTTCCCACAAGTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606180713_0oi8 CGGGCTGTATTTAAAGAGCCATACTCAAGAAG(+AAAAAA)GTACCAAGTCTGCGCAACCACACTGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606180711_9mt4 TCTGGGCTCTGCAGTGTGGTTGCGCAGACTTG(+AAAAAA)GTACCTTCTTGAGTATGGCTCTTTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606180710_fnih CTACTTAGGCCGTCAACTACAGGGAGAAGATG(+AAAAAA)GTACCCTGCCAGAGTGGGAGGGAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606180708_ogwj CTAAGCTCTCTCCTCCCTCCCACTCTGGCAGG(+AAAAAA)GTACCATCTTCTCCCTGTAGTTGACGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606180706_1em5 CCAGATGAAGCCCTTGGGGAACATCTGCCAGG(+AAAAAA)GATCCGCATGGTGTTGGCTCCATCCATTGCT NGG 10 8 17 8 24 0 100 5 10